The botanists study the internal structure of plants mainly by examining their cross section under a microscope. These thin slices can tell us a lot about the structure of the cells that make up the plant and how they vary in different parts of the plant. In 1665, a scientist named Robert Hooke looked at a piece of cork (a material from the thick outer part of certain trees) under a microscope and saw that it was made up of many tiny compartments. He named them cells and this term has been in use since then. The equipments needed for obtaining the sections include a sharp razor, a small fine brush and a number of watch glasses and microscopic slides. The razor is stroked across the top towards the body, cutting off thin slices as required. Cross-sections as well as the longitudinal pieces are obtained in the same manner. To obtain best results the razor and the material must be kept moist with water in case of fresh material or alcohol if the specimen is a preserved one. To prevent shrinkage the sections shaved off are 
brushed into water or alcohol. For quick examination the sections are placed on a slide with a drop of glycerine. The thin ones that show the cells clearly can be stained for permanent use. In fact staining is a process of adding dyes to show the different tissues in different colours. Many stains are dissolved in alcohol and before staining the sections must be placed in alcohol. After a certain period in the stain(s) the section is transferred to a series of watch glasses full of alcohol. This removes water and the excess stain. The alcohol is removed by dipping the sections in clove oil or benzene. The section is then placed on a clean glass slide with a drop of Canada Balsam (a resinous glue). A thin glass is added as a cover-slip and sealed by warming the balsam to harden it. The slide, properly labelled, can then be kept and examined whenever necessary. Details of time exposure for staining varies with the stain and material used. The information can be collected from a text-book or worked out by practice. In laboratory analysis many modern techniques are being adopted for in-depth studies on the subject.